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The best fluorophores for PALM imaging

Photoactivated Localization Microscopy, or PALM imaging, is a super-resolution technique that relies on the random activation of fluorophores to spatially resolve molecular details (Betzig et al. 2006). In brief, upon activation with the adequate laser, a sparse number of ...

Topics: Technical, Microscopy

Using dSTORM to study purified extracellular vesicles

Cells release different types of small-sized membrane vesicles (from 30nm to 1000nm) as a way of communicating with other cells and remove unwanted cell contents; these vesicles are collectively known as extracellular vesicles (EVs). Here we share how we have optimized the ...

Topics: Technical, Microscopy

Our 9 tips to optimize your immunofluorescence staining

We have all been there, after spending a day or two fixing and staining samples, the images don't look as good as expected, the signal is not optimal, and no obvious conclusion can be drawn from the data. We have put together some tips to help you optimize your ...

Topics: Technical

Beginner's guide to STORM buffers

When preparing a sample for STORM microscopy, one of the most important factors to consider is the choice of fluorophore. The ability to accurately localize single molecules is dependent on the use of fluorescent molecules that stochastically switch between a fluorescent “on” ...

Topics: Technical