The ONI Blog

The best fluorophores for PALM imaging

Posted by Anna Caballe on 27 Jun 2019

Photoactivated Localization Microscopy, or PALM imaging, is a super-resolution technique that relies on the random activation of fluorophores to spatially resolve molecular details (Betzig et al. 2006). In brief, upon activation with the adequate laser, a sparse number of fluorophores emit for a short period before bleaching. Because fluorophores are only activated at the same time in small numbers until all have emitted, it is possible to localize and track single molecules over time.

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Topics: Technical, Microscopy

Using dSTORM to study purified extracellular vesicles

Posted by Andras Miklosi on 11 Jun 2019

Cells release different types of small-sized membrane vesicles (from 30nm to 1000nm) as a way of communicating with other cells and remove unwanted cell contents; these vesicles are collectively known as extracellular vesicles (EVs). Here we share how we have optimized the imaging of purified EVs to study their size, content and behavior using super-resolution microscopy.

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Topics: Technical, Microscopy