Cells release different types of small-sized membrane vesicles (from 30nm to 1000nm) as a way of communicating with other cells and remove unwanted cell contents; these vesicles are collectively known as extracellular vesicles (EVs). Here we share how we have optimized the imaging of purified EVs to study their size, content and behavior using super-resolution microscopy.
We're excited to reveal more about a day in the life here at ONI. This week we're talking to one of our Optical Production Technicians - Natalia
Introducing ONIee of the week
Name: Natalia Najman
Title: Optical Production Technician
Joined: March 2017
We have all been there, after spending a day or two fixing and staining your samples, the images don't look as good as expected, the signal is not optimal, and no obvious conclusion can be drawn from the data. We have put together some tips to help you optimize your immunofluorescence staining protocol and increase the signal-to-noise ratio in your samples. If you are unsure about how the technique works, we recommend you read our immunofluorescence guide first.
The Science Innovation Union (SIU) at the University of Oxford hosted their first ever Art in Science Competition recently, with the aim to connect diverse scientific communities and provide scientists the chance of showcasing their research in the form of artwork. Sponsors, ONI, were at the award ceremony that took place on 10th May at the Department of Earth Science, University of Oxford.
The International Society for Extracellular Vesicles (ISEV) annual conference took place in Kyoto from 24-28th April 2019. On behalf of the ONI team that had the pleasure to attend, I summarize here my impressions from the meeting, how the extracellular vesicles (EV) data generated at ONI was received and the latest developments taking place in the EV field.
When preparing a sample for STORM microscopy, one of the most important factors to consider is the choice of fluorophore. The ability to accurately localize single molecules is dependent on the use of fluorescent molecules that stochastically switch between a fluorescent “on” state and a dark “off” state – a photophysical behavior that is often referred to as blinking.
The Biophysical Society (BPS) Annual Meeting brings together scientists working on a wide range of topics and attracts participants across different disciplines like physics, biology, chemistry, and computational sciences. The BPS annual meeting, therefore, offers a unique opportunity to engage in interesting conversations and get exposed to scientific questions from various perspectives. We also find that it is a great place for interdisciplinary interactions, especially between biologists and physicists, which leads to a better understanding of each other’s needs and research challenges.
We're excited to reveal more about a day in the life here at ONI. This week we're talking to one of our Field Applications Specialists.
On Sunday you may have seen that ONI made it into The Sunday Times’ Top 10 Disruptors to watch. It’s a big deal for the team here - our hard work has paid off. But most of all we’re very proud to be rubbing shoulders with the other companies that made it.