The ONI Blog

Using dSTORM to study purified extracellular vesicles

Posted by Andras Miklosi on 11 Jun 2019

Cells release different types of small-sized membrane vesicles (from 30nm to 1000nm) as a way of communicating with other cells and remove unwanted cell contents; these vesicles are collectively known as extracellular vesicles (EVs). Here we share how we have optimized the imaging of purified EVs to study their size, content and behavior using super-resolution microscopy.

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Topics: Technical, Microscopy

ONIee of the week - Optical Production Technician

Posted by Chris Best on 11 Jun 2019

We're excited to reveal more about a day in the life here at ONI. This week we're talking to one of our Optical Production Technicians - Natalia 

 

Introducing ONIee of the week

Name:           Natalia Najman

Born:             Poland

Title:             Optical Production Technician 

Joined:         March 2017      

 

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Topics: People

Our 9 tips to optimize your immunofluorescence staining

Posted by Anna Caballe on 15 May 2019

We have all been there, after spending a day or two fixing and staining your samples, the images don't look as good as expected, the signal is not optimal, and no obvious conclusion can be drawn from the data. We have put together some tips to help you optimize your immunofluorescence staining protocol and increase the signal-to-noise ratio in your samples. If you are unsure about how the technique works, we recommend you read our immunofluorescence guide first.

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Topics: Technical

The Art in Science Competition awards from SIU with ONI

Posted by Anna Caballe on 15 May 2019

The Science Innovation Union (SIU) at the University of Oxford hosted their first ever Art in Science Competition recently, with the aim to connect diverse scientific communities and provide scientists the chance of showcasing their research in the form of artwork. Sponsors, ONI, were at the award ceremony that took place on 10th May at the Department of Earth Science, University of Oxford. 

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Topics: Events

Impressions on ISEV2019: when the world of EVs met in Kyoto

Posted by Valerio Pereno on 10 May 2019

The International Society for Extracellular Vesicles (ISEV) annual conference took place in Kyoto from 24-28th April 2019. On behalf of the ONI team that had the pleasure to attend, I summarize here my impressions from the meeting, how the extracellular vesicles (EV) data generated at ONI was received and the latest developments taking place in the EV field.

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Topics: Events

Beginner's guide to STORM buffers

Posted by Mark Harmon on 28 Mar 2019

 When preparing a sample for STORM microscopy, one of the most important factors to consider is the choice of fluorophore. The ability to accurately localize single molecules is dependent on the use of fluorescent molecules that stochastically switch between a fluorescent “on” state and a dark “off” state – a photophysical behavior that is often referred to as blinking.

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Topics: Technical

BPS19 – The best of two worlds: where biology meets physics

Posted by Linnéa Olofsson on 21 Mar 2019

The Biophysical Society (BPS) Annual Meeting brings together scientists working on a wide range of topics and attracts participants across different disciplines like physics, biology, chemistry, and computational sciences. The BPS annual meeting, therefore, offers a unique opportunity to engage in interesting conversations and get exposed to scientific questions from various perspectives. We also find that it is a great place for interdisciplinary interactions, especially between biologists and physicists, which leads to a better understanding of each other’s needs and research challenges.

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Topics: Events

ONIee of the week - Field Applications Specialist

Posted by Chris Best on 19 Mar 2019

We're excited to reveal more about a day in the life here at ONI. This week we're talking to one of our Field Applications Specialists.

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Topics: People

Making the Sunday Times Top 10 #Disruptors: What connects us?

Posted by Stuart Wilson on 6 Dec 2018

On Sunday you may have seen that ONI made it into The Sunday Times’ Top 10 Disruptors to watch. It’s a big deal for the team here - our hard work has paid off. But most of all we’re very proud to be rubbing shoulders with the other companies that made it.

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Topics: People