Photoactivated Localization Microscopy, or PALM imaging, is a super-resolution technique that relies on the random activation of fluorophores to spatially resolve molecular details (Betzig et al. 2006). In brief, upon activation with the adequate laser, a sparse number of fluorophores emit for a short period before bleaching. Because fluorophores are only activated at the same time in small numbers until all have emitted, it is possible to localize and track single molecules over time.
We have all been there, after spending a day or two fixing and staining your samples, the images don't look as good as expected, the signal is not optimal, and no obvious conclusion can be drawn from the data. We have put together some tips to help you optimize your immunofluorescence staining protocol and increase the signal-to-noise ratio in your samples. If you are unsure about how the technique works, we recommend you read our immunofluorescence guide first.
The Science Innovation Union (SIU) at the University of Oxford hosted their first ever Art in Science Competition recently, with the aim to connect diverse scientific communities and provide scientists the chance of showcasing their research in the form of artwork. Sponsors, ONI, were at the award ceremony that took place on 10th May at the Department of Earth Science, University of Oxford.